Focus on chemical reactions to tag cancer therapy for detection within tumors and in the central nervous system

Coordinator: Castel, H

Background: Little information is available concerning the ability of chemotherapy and targeted therapy to cross the blood-brain barrier. In humans, post-treatment cognitive deficits have been described with alteration of activity in some brain areas involved in memory and attention. However, the direct penetration of therapies in these brain areas has not been evaluated.

Objectives: Our study focused on an original chemical grafting reaction to improve camptothecin and SN-38 coupling to FITC, maintain their therapeutic efficacy, and initially monitor tumours targeted in vitro and in vivo.

Study methodology: Bio-orthogonal chemical grafting reaction of FITC onto the camptothecin molecule and molecular and cellular validation of the targeting and therapeutic efficacy towards glioblastoma cell lines and a xenograft model of U87 glioblastoma line in Nude mice.

Current study status: closed

Collaboration: COBRA Laboratory

Sponsor: CNRS, Inserm and the Université de Rouen